Linking - Rhubarb
Information Portal (U.S. National Library of Medicine) - Chrysophanic acid
(Kyoto Encyclopedia of Genes and Genomes) - Chrysophanol
is a member of the anthraquinone family and has multiple
pharmacological effects, but the exact mechanism of
the anti-inflammatory effects of chrysophanol has yet
to be thoroughly elucidated. In this study, we attempted
to determine the effects of chrysophanol on dextran
sulfate sodium (DSS)-induced colitis and lipopolysaccharide
(LPS)-induced inflammatory responses in mouse peritoneal
macrophages. The findings of this study demonstrated
that chrysophanol effectively attenuated overall clinical
scores as well as various pathological markers of colitis.
Additionally, chrysophanol inhibited the production
of tumor necrosis factor (TNF)-a, interleukin (IL)-6
and the expression of cyclooxygenase (COX)-2 levels
induced by LPS. We showed that this anti-inflammatory
effect of chrysophanol is through suppression of the
activation of NF-kB and caspase-1 in LPS-stimulated
macrophages. These results provide novel insights into
the pharmacological actions of chrysophanol as a potential
molecule for use in the treatment of inflammatory diseases.
a well-known Chinese herbal medicine, is also used in
Europe and other places of the world. Anthraquinones
derivatives are thought to be the major active components.
A pressurized liquid extraction (PLE) and capillary
zone electrophoresis (CZE) separation were developed
for simultaneous determination of five anthraquinones
including aloe-emodin, emodin, chrysophanol, physcion,
and rhein in Rhubarb. The effects of the experimental
variables on PLE and CZE have been optimized. The optimum
conditions of PLE were: solvent, methanol; temperature,
1407C; particle size, 0.13–0.2 mm; static extraction
time, 5 min; pressure, 1500 psi; and one extraction.
The best separation of the five anthraquinones could
be obtained using 50 mM borate buffer (pH 8.2) containing
25% isopropyl alcohol and 25% acetontrile as modifier,
while the separation voltage was 25 kV and the temperature
was at 207C. The method developed is accurate, simple,
and reproducible, and could be used for quality control
of Rhubarb and its medical preparations.