N,N-BIS(2-HYDROXYETHYL)GLYCINE

PRODUCT IDENTIFICATION
CAS NO. 150-25-4

N,N-BIS(2-HYDROXYETHYL)GLYCINE
EINECS NO. 205-755-1
FORMULA (HOCH2CH2)2NCH2COOH
MOL WT. 163.17
H.S. CODE  
TOXICITY  
SYNONYMS Bicine; Diethylolglycine; DHEG; N,N-Di-(Hydroxyethyl) glycine;
DERIVATION

 

CLASSIFICATION

 

PHYSICAL AND CHEMICAL PROPERTIES
PHYSICAL STATE white crystalline powder
MELTING POINT 190 - 192 C
BOILING POINT  
SPECIFIC GRAVITY  
SOLUBILITY IN WATER Soluble (slightly soluble in ethanol, ether, chloroform and benzene)
AUTOIGNITION  
pH  
VAPOR DENSITY  
NFPA RATINGS Health: 1; Flammability: 0; Reactivity: 0

REFRACTIVE INDEX

  
FLASH POINT

 
STABILITY Stable under ordinary conditions. Hygroscopic.

GENERAL DESCRIPTION & APPLICATIONS
Buffer is a substance, generally a solution, that can keep its pH constant, despite the addition of strong acids or strong bases and external influences of temperature, pressure, volume, redox potential. Buffer prevents change in the concentration of another chemical substance, e.g., proton donor and acceptor systems that prevent marked changes in hydrogen ion concentration (pH). Many acid-base reactions take place in living organisms. However, for organisms to perform certain vital functions, the body fluids associated with these functions must maintain a constant pH. For example, blood must maintain a pH of close to 7.4 in order to carry oxygen from the lungs to cells; blood is therefore a powerful buffer. The commonest buffer in chemical solution systems is the acid-base buffer.
  • Bicarbonate buffer; a buffer system composed of bicarbonate ions and dissolved carbon dioxide; in the body, this system is an important factor in determining the pH of the blood as the concentration of bicarbonate ions is regulated by the kidneys and of carbon dioxide by the respiratory system.
  • Cacodylate buffer; one containing an organic arsenical salt, used in preparing fixatives for electron microscopy.
  • Phosphate buffer,   a buffer system composed of KH2PO4 and Na2HPO4; in the body, it is important in regulating the pH of the renal tubular fluids; when 0.025 molal (equimolal of the potassium and sodium salts), the pH is 6.865 at 25 C.
  • Protein buffer,   a buffer system involving proton donor and proton acceptor groups of the amino acid residues of proteins.
  • TRIS buffer (tromethamine): an amine base used intravenously as an alkalizer for the correction of metabolic acidosis. The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per C temperature decrease, and decreases 0.03-0.05 unit per ten-fold dilution.
  • Veronal buffer; a barbital buffer commonly used in the preparation of fixatives for electron microscopy.
Bicine [N,N-Bis(2-hydroxyethyl)glycine] is a general purpose buffer for biological research. Useful pH range is 7.6 - 9.0. Its applications include:
  • Tissue culture
  • Phosphorylation and Photophosphorylation
  • Fixative Transmission Electron Microscopy
  • Protein synthesis
  • Preventing binding to non-receptor materials
SALES SPECIFICATION

APPEARANCE

 white crystalline powder
ASSAY

99.0% min

Cl

50ppm max

SO4

50ppm max

Cu

2ppm max

Fe

3ppm max

Pb

5ppm max

pH

 4 - 5 (10% sol. at 25 C)
TRANSPORTATION
PACKING 1kgs, 25kgs in fiber drum
HAZARD CLASS Not regulated
UN NO.

 
OTHER INFORMATION
Hazard Symbols: , Risk Phrases: , Safety Phrases: 22-24/25

BIOLOGICAL BUFFERS

Buffers

CAS #

Useful pH Range

pKa
N-(2-Acetamido)-2-aminoethanesulfonic acid

ACES

7365-82-4

6.1 - 7.5

6.8
N-(2-(Acetamido)imino)diacetic acid

ADA

26239-55-4

6.0 - 7.2

6.59
2-Amino-2-methyl-1-propanol

AMP

124-68-5

9.0 - 10.5

9.70
2-Amino-2-methyl-1,3-propanediol

AMPD

115-69-5

7.8 - 9.7

8.80
N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid

AMPSO

68399-79-1

8.3 - 9.7

9.00
N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid

BES

10191-18-1

6.4 - 7.8

7.09
N,N-Bis(2-hydroxyethyl)glycine

Bicine

150-25-4

7.6 - 9.0

8.3
2,2'-(Propane-1,3-diyldiimino)bis[2-(hydroxymethyl)propane-1,3-diol]

Bis-Tris Propane

64431-96-5

6.3 - 9.5

(1) 6.8, (2) 9.0
2-[Bis(2-hydroxyethyl)imino]-2-(hydroxymethyl)-1,3-propanediol

Bis-Tris

6976-37-0

5.8 - 7.2

6.50
2-[Bis(2-hydroxyethyl)imino]-2-(hydroxymethyl)-1,3- propanediol Hydrochloride

BIS-TRIS HCl

124763-51-5

5.8 - 7.2

6.50
4-(Cyclohexylamino)-1-butanesulfonic acid

CABS

161308-34-5

10.0 - 11.4

10.70
3-(Cyclohexylamino)-1-propanesulfonic acid

CAPS

1135-40-6

9.7 - 11.1

10.40
3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid

CAPSO

73463-39-5

8.9 - 10.3

9.60
2-(Cyclohexylamino)ethanesulfonic acid

CHES

103-47-9

8.6 - 10.0

9.49
3-[N-Bis(hydroxyethyl)amino]-2-hydroxypropanesulfonic acid

DIPSO

68399-80-4

7.0 - 8.2

7.60
4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid

EPPS

16052-06-5

7.3 - 8.7

8.00
N-Glycylglycine

Gly-Gly

556-50-3

7.5 - 8.9

8.20
N-(2-Hydroxyethyl)piperazine-N'-(4-butanesulfonic acid)

HEPBS

161308-36-7

7.6 - 9.0

8.30
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid

HEPES

7365-45-9

6.8 - 8.2

7.55
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid Hemisodium salt

HEPES Hemisodium

103404-87-1

8.2

7.55
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid Potassium salt

HEPES potassium

82207-62-3

6.8 - 8.2

7.55
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid Sodium salt Hydrate

HEPES Sodium Hydrate

75277-39-3

6.8 - 8.2

7.5

Imidazole

Imidazole

288-32-4

6.2 - 7.8

 
2-(4-Morpholino)ethanesulfonic acid

MES

4432-31-9

5.5 - 6.7

6.2
2-(4-Morpholino)ethanesulfonic acid Sodium salt

MES sodium

71119-23-8

5.5 - 6.7

6.2

MOBS

115724-21-5

6.9 - 8.3

7.60
3-N-Morpholino propansulfonic acid

MOPS

1132-61-2

6.5 - 7.9

7.2
3-N-Morpholino propansulfonic acid Hemisodium salt

MOPS Hemisodium

117961-20-3

6.5 - 7.9

7.2
3-N-Morpholino propansulfonic acid Sodium salt

MOPS Sodium

71119-22-7

6.5 - 7.9

7.2
3-Morpholino-2-hydroxypropanesulfonic acid

MOPSO

68399-77-9

6.2 - 7.6

6.90
Piperazine-N,N'-bis(2-ethanesulfonic acid)

PIPES

5625-37-6

6.1 - 7.5

6.8
Piperazine-N,N'-bis(2-ethanesulfonic acid) Dipotassium salt

PIPES Dipotassium

108321-27-3

6.1 - 7.5

6.8
Piperazine-N,N'-bis(2-ethanesulfonic acid) Disodium salt

PIPES Disodium

76836-02-7

6.1 - 7.5

6.8
Piperazine-N,N'-bis(2-hydroxypropanesulfonic acid

POPSO

68189-43-5

7.2 - 8.5

7.80
N-Tris(Hydroxymethyl)methyl-4-aminobutanesulfonic acid

TABS

54960-65-5

8.2 - 9.6

8.90
N-(Tris(hydroxymethyl)methyl)-3-aminopropanesulfonic acid

TAPS

29915-38-6

7.7 - 9.1

8.40
N-[Tris(hydroxymethyl)methyl]-3-amino-2-hydroxypropanesulfonic acid

TAPSO

68399-81-5

7.0 - 8.2

7.60
Triethanolamine

TEA

102-71-6

7.3 - 8.3

7.80
N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid

TES

7365-44-8

6.8 - 8.2

7.40
N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid hemisodium salt

TES hemisodium

 

6.8 - 8.2

7.5
N-(Tris(hydroxymethyl)methyl)glycine

Tricine

5704-04-1

7.4 - 8.8

8.2
Tris(hydroxymethyl)methylamine

Tris

77-86-1

7.0 - 9.1

8.06
Tris(hydroxymethyl)methylamine acetate

Tris Acetate

6850-28-8

7.0 - 9.01

8.06
Tris(hydroxymethyl)methylamine Hydrochloride

Tris Hydrochloride

1185-53-1

7.0 - 9.01

8.06