3,3',5,5'-TETRAMETHYLBENZIDINE

Synonyms. 3,3',5,5'-Tetramethylbenzidine; TMB; 3,3',5,5'-Tetramethyl-(1,1'-biphenyl)-4,4'-diamine; 3,5,3',5'-Tetramethylbenzidine; 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl; 4-(4-Amino-3,5-dimethylphenyl)-2,6-dimethylaniline; 3,3',5,5'-Tetramethylbenzidine substrate;

3,3',5,5'-TETRAMETHYLBENZIDINE

Click for original image

 

PRODUCT IDENTIFICATION

CAS RN

54827-17-7 (parent), 207738-08-7 (dihydrochloride hydrate)

EINECS RN

259-364-6

FORMULA

 [C6H2(CH3)2-4-NH2]2

MOLE WEIGHT

240.34

H.S CODE

 2921.59.4000

SMILES

 c1(c(cc(c2cc(c(N)c(c2)C)C)cc1C)C)N

CLASSIFICATION

Chromogenic, Indicator

EXTRA NOTES

Chromogenic Compounds: Colorless, endogenous or exogenous pigment precursors that may be transformed by biological mechanisms into colored compounds; used in biochemical assays and in diagnosis as indicators, especially in the form of enzyme substrates. Synonym: chromogens (not to be confused with pigment-synthesizing bacteria also called chromogens).[MeSH]

 

PHYSICAL AND CHEMICAL PROPERTIES

PHYSICAL STATE

white to yellowish crystalline powder

MELTING POINT

166 ~ 171 C (Decomposes)

BOILING POINT

 

DENSITY

 

SOLUBILITY IN WATER

8.2 mg/l

SOLVENT SOLUBILITY

Soluble in methanol

VAPOR DENSITY

 

log P(octanol-water)

 4.11

VAPOR PRESSURE

 5.00E-07 mm Hg

AUTOIGNITION TEMP

  
pK

 

REFRACTIVE INDEX

 

FLASH POINT

 

 

STABILITY AND REACTIVITY
STABILITY Stable under normal conditions.

INCOMPATIBLE MATERIALS

Metals, Strong acids, Strong oxidizing agents.

POLYMERIZATION

Has not been reported

NFPA RATINGS

Health: 1, Flammability: 1, Reactivity: 0

 

EXTERNAL LINKS & GENERAL DESCRIPTION

USA.gov - 3,3',5,5'-Tetramethylbenzidine

Wikipedia Linking - 3,3',5,5'-Tetramethylbenzidine

Google Scholar Search - 3,3',5,5'-Tetramethylbenzidine

U.S. National Library of Medicine - 3,3',5,5'-Tetramethylbenzidine

PubChem Compound Summary - 3,3',5,5'-Tetramethylbenzidine

NCBI (http://www.ncbi.nlm.nih.gov/) -  3,3',5,5'-Tetramethylbenzidine

Material Safety Data Sheet - 3,3',5,5'-Tetramethylbenzidine

Hazardous Substances Data Bank - 3,3',5,5'-Tetramethylbenzidine

EPA - Substance Registry Services - 3,3',5,5'-Tetramethylbenzidine

http://www.sigmaaldrich.com/
Application: 3,3',5,5'-Tetramethylbenz​idine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases

 

SALES SPECIFICATION

APPEARANCE

yellowish crystalline powder

ASSAY

98.0% min

LOSS ON DRYING

5.0% max

SULFATED ASH

0.5% max

 

TRANSPORT & REGULATORY INFORMATION

UN NO.

 

HAZARD CLASS

 

PACKING GROUP  

 

SAFETY INFORMATION

HAZARD OVERVIEW

GHS (Globally Harmonised System) Classification: Skin irritation. Eye irritation. Specific target organ toxicity - single exposure. Hazard statements: Harmful if inhaled. Suspected of causing genetic defects. Harmful if swallowed. May cause damage to organs. Harmful in contact with skin.

SIGNAL WORD Warning

PICTOGRAMS

PICTOGRAM_GHS07_exclamation.jpgPICTOGRAM_GHS08_health.jpg

HAZARD STATEMENTS

H302-H312-H332-H341-H371

P STATEMENTS

P280-P281-P302 + P352-P260-P301+ P312-P304 + P340

EC DIRECTIVES

 

HAZARD CODES

CODE_Xn_Xi_harmful_Irritant.gif

RISK PHRASES

20/21/22-36/37/38-68

SAFETY PHRASES

26-36

 

PACKING

Preserved in light-resistant and well-closed bottles

 

PRICE INFORMATION