PRODUCT IDENTIFICATION

CLASSIFICATION

Buffer

EXTRA NOTES

PHYSICAL AND CHEMICAL PROPERTIES

REFRACTIVE INDEX

EXTERNAL LINKS & GENERAL DESCRIPTION

Wikipedia Linking

Google Scholar Search

Drug Information Portal (U.S. National Library of Medicine) - Bicine

PubChem Compound Summary - Bicine

http://www.ebi.ac.uk/chebi/ -  Bicine

http://www.ncbi.nlm.nih.gov/ -  Bicine

Material Safety Data Sheet

http://www.sigmaaldrich.com/
Recommended buffer for low temperature biochemical work. Used in the preparation of stable substrate solution for serum guanase determination. Spacer in plasma protein fractionation with Sephadex by isotachophoresis

Local:
Buffer is a substance, generally a solution, that can keep its pH constant, despite the addition of strong acids or strong bases and external influences of temperature, pressure, volume, redox potential. Buffer prevents change in the concentration of another chemical substance, e.g., proton donor and acceptor systems that prevent marked changes in hydrogen ion concentration (pH). Many acid-base reactions take place in living organisms. However, for organisms to perform certain vital functions, the body fluids associated with these functions must maintain a constant pH. For example, blood must maintain a pH of close to 7.4 in order to carry oxygen from the lungs to cells; blood is therefore a powerful buffer. The commonest buffer in chemical solution systems is the acid-base buffer.

• Bicarbonate buffer; a buffer system composed of bicarbonate ions and dissolved carbon dioxide; in the body, this system is an important factor in determining the pH of the blood as the concentration of bicarbonate ions is regulated by the kidneys and of carbon dioxide by the respiratory system.
• Cacodylate buffer; one containing an organic arsenical salt, used in preparing fixatives for electron microscopy.
• Phosphate buffer,   a buffer system composed of KH₂PO₄ and Na₂HPO₄; in the body, it is important in regulating the pH of the renal tubular fluids; when 0.025 molal (equimolal of the potassium and sodium salts), the pH is 6.865 at 25 C.
• Protein buffer,   a buffer system involving proton donor and proton acceptor groups of the amino acid residues of proteins.
• TRIS buffer (tromethamine): an amine base used intravenously as an alkalizer for the correction of metabolic acidosis. The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per C temperature decrease, and decreases 0.03-0.05 unit per ten-fold dilution.
• Veronal buffer; a barbital buffer commonly used in the preparation of fixatives for electron microscopy.

• Tissue culture
• Phosphorylation and Photophosphorylation
• Fixative Transmission Electron Microscopy
• Protein synthesis
• Preventing binding to non-receptor materials

APPEARANCE

99.0% min

Cl

50ppm max

SO4

50ppm max

Cu

2ppm max

Fe

3ppm max

Pb

5ppm max

pH